Running the HPLC
Adsorption of DNA plasmids by
inserting genetically engineered sequences
Use of DNA plasmids in future gene therapy or DNA vaccine
products is currently cost-prohibitive, mainly due to the
expensive chromatography steps necessary for final product
purification. The goal of this project is to explore a novel
and less expensive approach to traditional chromatography.
Small DNA sequences have been designed for insertion into
the plasmids, that the potential to adsorb to inexpensive
Expanded Bed Adsorption for
Recovery of Therapeutic Proteins
Expanded bed adsorption is attractive in the recovery of
bio-pharmaceuticals, because this single step can achieve
purification without the need/cost of a cell removal (i.e.
filtration) step. Experiments will be conducted to determine
conditions that minimize interaction between the cells and
resin, while maximizing the selective adsorption of the
protein bio-product on the resin. This is a collaborative
project with researchers at Glaxo-SmithKline.
Using CFD modeling to improve
animal cell culture steps
Fluent is a CFD software that is windows based, and
computes/displays flow in 3D geometries. The results of our
CFD model of oxygen transfer in a bioreactor are being
compared to actual experimental data from our laboratory.
The CFD model can then be used to determine the optimum
bioreactor configuration (i.e. sparger location etc…) for
given operating conditions (RPM, type cell line..).