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    Disassembling our CARR centrifuge

    Plasmid DNA: Clinical trials for gene therapy products began in the 1990s. Because of the safety issues surrounding the use of viral vectors, DNA plasmids are being considered as gene vectors With plasmid-mediated gene therapy, a medicinal gene, carried by a double stranded DNA plasmid, is administered to the patient. The gene codes for a protein that is either missing/defective (i.e. gene therapy) or is antigenic (i.e. DNA vaccine). Use of DNA plasmids in future gene therapy or DNA vaccine products is currently cost-prohibitive, mainly due to the expensive chromatography steps necessary for final product purification. The goal of this research is to explore novel and less expensive approaches to traditional chromatography.

    CHO cells: Chinese Hamster Ovary Cells are a popular mammalian cell line used in the biopharmaceutical industry to make monoclonal antibodies. These cells are damaged by chemical and physical stresses during cell culture and harvesting. The goal of this research is to understand the degree to which bioreactor, centrifuge and filter design and operating conditions effect the damage of these cells.