Disassembling our CARR centrifuge
Plasmid DNA: Clinical trials for gene therapy products began
in the 1990’s. Because of the safety issues surrounding the use of viral
vectors, DNA plasmids are being considered as gene vectors With
plasmid-mediated gene therapy, a medicinal gene, carried by
a double stranded DNA plasmid, is administered to the patient.
The gene codes for a protein that is either missing/defective
(i.e. gene therapy) or is antigenic (i.e. DNA vaccine). Use
of DNA plasmids in future gene therapy or DNA vaccine products
is currently cost-prohibitive, mainly due to the expensive chromatography
steps necessary for final product purification. The goal of
this research is to explore novel and less expensive approaches
to traditional chromatography.
CHO cells: Chinese Hamster Ovary Cells are a popular mammalian
cell line used in the biopharmaceutical industry to make monoclonal
antibodies. These cells are damaged by chemical and physical
stresses during cell culture and harvesting. The goal of this
research is to understand the degree to which bioreactor, centrifuge
and filter design and operating conditions effect the damage
of these cells.